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The study of vertical mobility of heavy metals and their transfer to mushrooms
Jurnečka, Roman ; Doležalová Weissmannová, Helena (referee) ; Komendová, Renata (advisor)
This bachelor thesis deals with the issue of contamination of forest soils and edible mushrooms with lead, cadmium, copper and zinc in the Jeseníky Mountains, specifically in the Domašov and Vápenná forests. Sampling took place at 12 localities, divided into spruce and beech stands. Atomic absorption spectrometry AAS ContrAA 800D was chosen to determine the content of selected heavy metals. Based on the measured values of the concentrations of the studied metals and other soil properties, dependences were created to determine the vertical mobility of selected metals in the soil profile. Vertical mobility depends on the physico-chemical properties of the soil (pH, C and S content). The dependence of the content of selected heavy metals in the soil on the pH value of the soil was found. In the case of lead, the sorption capacity of the soil organic component was confirmed. The risk and above-limit content of Pb, Cd and Cu was found on the yellow-boletus mushroom. Cadmium shows the highest bioavailability. Lead was evaluated as the element with the lowest mobility.
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The study of contamination of forested areas of the Czech Republic by mercury
Trávníčková, Gabriela ; Řezáčová, Veronika (referee) ; Komendová, Renata (advisor)
This bachelor thesis deals with the issue of mercury in the environment. It focuses on determining the concentration of total mercury in edible mushrooms and in the soil in 12 localities in the Jeseníky Mountains. A single-purpose absorption spectrometer AMA 254 was used to determine the mercury content. The sites where sampling took place were divided into 6 sites with spruce stand and 6 sites with beech stand. No relationship was found between the type of stand and the amount of accumulated mercury in the mushrooms. On a representative sample of yellow-boletus mushrooms found in each locality, it was found that the higher the amount of mercury in the mushroom, the lower the amount of mercury in the soil. The fungi are therefore able to accumulate mercury from the environment. Thus, they can become a potential source of intoxication when ingested. The mobility of heavy metals in the soils depends on many soil properties (pH, carbon and sulfur content). Metals form more soluble compounds in lower pH environments. The soil pH was very low and the lower the soil pH, the higher mercury concentration in the soil. This fact could be supported by acid fallout. The relationship between the mercury content and the carbon content of the soil confirmed that carbon has the ability to bind mercury. The higher the amount of carbon in the soil, the higher the amount of mercury.
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Determination of selected metals in soils and mushrooms in the vicinity of the D1 highway.
HORÁKOVÁ, Tereza
The aim of the bachelor thesis was to determine the concentrations of heavy metals in edible mushroom samples and underlying soils. The elements measured were mainly copper, iron and zinc, followed by manganese, strontium and rubidium. The literature search contains brief information on each element and their content in soils and fruiting bodies of mushrooms in other studies. The theoretical part also includes chapters on fungi, soils and their contamination, the effect of vehicular traffic on the heavy metal content of soils and the used analytical method, which was atomic absorption spectrometry. For the practical part, 24 samples of eight different species of edible mushrooms were collected in the autumn of 2020 and 2021 in the vicinity of the D1 motorway. As part of the collection, the soil beneath the mushroom was also sampled to a depth of approximately 15 cm. The samples were first perfectly dried, then processed by microwave digestion, and finally analysed by AAS. All work was carried out in the laboratories of the Department of Applied Chemistry, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice. The results of the measurements were tabulated and processed statistically and graphically. Finally, they were evaluated in a discussion. The analysis showed that birch and birch goosefoot accumulate the least heavy metals in their fruiting bodies. The spruce budworm, the pink toadflax, the celandine and the brown mushroom accumulate metals on a large scale. In total, 4.2-45 mg.kg-1 dry weight Cu, 97,4-4166 mg.kg-1 Fe, 52,1-338,7 mg.kg-1 Zn, 3-61,4 mg.kg-1 Mn, 1,3-7 mg.kg-1 Sr and 7,7-300,9 mg.kg-1 Rb were measured in mushrooms. In soils, the values were as follows: 5,2-78,6 mg.kg-1 Cu, 2 965-41 983 mg.kg-1 Fe, 25,8- 174,2 mg.kg-1 Zn, 49,7-1 868 mg.kg-1 Mn, 6,2-25,2 mg.kg-1 Sr and 12,2-114,5 mg.kg-1 Rb in dry weight. These results show that strontium was the least represented heavy metal studied and iron was the most represented.
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The study of vertical mobility of heavy metals and their transfer to mushrooms
Jurnečka, Roman ; Doležalová Weissmannová, Helena (referee) ; Komendová, Renata (advisor)
This bachelor thesis deals with the issue of contamination of forest soils and edible mushrooms with lead, cadmium, copper and zinc in the Jeseníky Mountains, specifically in the Domašov and Vápenná forests. Sampling took place at 12 localities, divided into spruce and beech stands. Atomic absorption spectrometry AAS ContrAA 800D was chosen to determine the content of selected heavy metals. Based on the measured values of the concentrations of the studied metals and other soil properties, dependences were created to determine the vertical mobility of selected metals in the soil profile. Vertical mobility depends on the physico-chemical properties of the soil (pH, C and S content). The dependence of the content of selected heavy metals in the soil on the pH value of the soil was found. In the case of lead, the sorption capacity of the soil organic component was confirmed. The risk and above-limit content of Pb, Cd and Cu was found on the yellow-boletus mushroom. Cadmium shows the highest bioavailability. Lead was evaluated as the element with the lowest mobility.
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The study of contamination of forested areas of the Czech Republic by mercury
Trávníčková, Gabriela ; Řezáčová, Veronika (referee) ; Komendová, Renata (advisor)
This bachelor thesis deals with the issue of mercury in the environment. It focuses on determining the concentration of total mercury in edible mushrooms and in the soil in 12 localities in the Jeseníky Mountains. A single-purpose absorption spectrometer AMA 254 was used to determine the mercury content. The sites where sampling took place were divided into 6 sites with spruce stand and 6 sites with beech stand. No relationship was found between the type of stand and the amount of accumulated mercury in the mushrooms. On a representative sample of yellow-boletus mushrooms found in each locality, it was found that the higher the amount of mercury in the mushroom, the lower the amount of mercury in the soil. The fungi are therefore able to accumulate mercury from the environment. Thus, they can become a potential source of intoxication when ingested. The mobility of heavy metals in the soils depends on many soil properties (pH, carbon and sulfur content). Metals form more soluble compounds in lower pH environments. The soil pH was very low and the lower the soil pH, the higher mercury concentration in the soil. This fact could be supported by acid fallout. The relationship between the mercury content and the carbon content of the soil confirmed that carbon has the ability to bind mercury. The higher the amount of carbon in the soil, the higher the amount of mercury.
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The content of selected group 1 and 2 metals in edible mushrooms
MÁCHOVÁ, Nikola
Edible mushrooms are able to accumulate elements, especially some heavy metals and radionuclides, in their fruiting body. This work engages in accumulation of chosen metals (Groups 1 and 2) in basidiomycete fungi and soil. The first picking happened in autumn 2017. Eleven mushroom specimens were found - several fruiting bodies of each type. The soil where the mushrooms had grown was also taken. During the other picking in autumn 2018 ten types of mushrooms were taken. The locality in the Czech Republic (Ledenice) was chosen, because these fungi were frequently found there: Agaricus campestris, Boletus edulis, Suillus variegatus, Xerocomellus chrysenteron, Neoboletus luridiformis, Leccinum rufum, Macrolepiota procera, Suillus luteus, Imleria badia, Leccinum scabrum, Lactarius deliciosus, Agaricus silvaticus, Suillus bovinus and Amanita rubescens. The chosen metals were Lithium (Li), Strontium (Sr), Cesium (Cs) and Rubidium (Rb). I used methods of atomic absorption spectrometry (AAS). In both analysis (2017 and 2018) the largest content of Lithium was recorded in Agaricus campestris fruiting body (2.3 mg/kg and 1.6 mg/kg) and the largest content of Strontium in Leccinum rufum (3.7 mg/kg and 6.6 mg/kg). It was shown that varieties of the Bolete family demonstrated the biggest accumulation of Rubidium (Boletus edulis 519 mg/kg, Neoboletus luridiformis 398.6 mg/kg). The biggest content of Cesium (about 1.1 mg/kg) was recorded also in this family.
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Contents of mercury in edible mushrooms growing in a historical silver-mining area
BÍLÁ, Eva
Contents of mercury were determined using AAS method (instrument AMA-254) in 109 fruiting body samples of 14 edible mushroom species. The mushrooms were collected from a forest of a historical area of silver mining. The highest mercury accumulation was observed in samples of Boletus aestivalis, Maccrolepiota procera and Boletus edulis. Mercury content was highly comparable with concentrations observed in unpolluted sites.
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Mercury contents in mushrooms growing nearby a reservoir Skalka
SZABOVÁ, Martina
Contents of mercury were determined using AAS method (instrument AMA-254) in 64 fruiting body samples of 15 edible mushroom species. The mushrooms were collected from a forest nearby a reservoir Skalka. The highest mercury accumulation was observed in a sample of Boletus edulis (15.5 mg/ kg dry matter). Mercury contents were highly comparable with concentrations observed in unpolluted sites. It can be therefore concluded that mercury contents in mushroom from the observed area do not represent any health risk.
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Mercury contents in mushrooms growing nearby a factory producing ammunition and an industrial shooting-range
BRÁZDOVÁ, Lenka
Contents of mercury were determined using AAS method (instrument AMA-254) in 119 fruiting body samples of 41 mostly edible mushroom species. The mushrooms were collected from a forest near by the ammunition factory and its industrial shooting-range (fy Sellier & Bellot, Vlašim, Czech Republic). The highest mercury accumulation was observed in samples of Agaricus arvensis, Lepista nuda, Calocybe gambosa and Russu-la nigricans. Mercury content was highly comparable with concentrations observed in unpolluted sites.
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Mercury contents in mushrooms growing nearby Horní Slavkov.
BAŽILOVÁ, Štěpánka
Contents of mercury were determined using AAS method (instrument AMA-254) in 130 fruiting body samples of 13 edible mushroom species. The mushrooms were collected from a forest of a historical area of tin mining. The highest mercury accumulation was observed in samples of Boletus edulis and Calocybe gambosa. Mercury content was highly comparable with concentrations observed in unpolluted sites.
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